New FISH probes using labeled oligonucleotides (ODNs) for 5 color chromosome enumeration in interphase nuclei.

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5 Minute Hybridization
Now available:
Probes for chromosomes X, Y, Yq12, 2, 3, 4, 6, 7, 8, 9q12, 10, 11, 12, 15, 16, 16q12, 17, 18, 20,
all telomeres and all centromeres.
Customized probe kits from 1 to 5 colors (4 visible 1 far red)

 
 

5 fluors available

Colors
Red (ex580/em599)
Aqua (ex418/em467)
Green (ex490/em516)
Orange (ex558/em572)
Far Red (ex653/em674)

 
 
 
 

Applications
PGD
Prenatal
Bone Marrow Transplant
 
 
 
 

Chromosomes and interphase nuclei hybridized with the 5 color kit. Chromosome Y in aqua, X in red, 15 in orange, 17 in green and 20 in magenta. Slides were counterstained with DAPI, represented by grey to avoid interactions with the other colors.
Left, peripheral blood; center, human embryo; and right, human sperm.

  

 

Other Technologies Available
BioLoop: Oligos with high density biotin labeling
AminoLoop: High density oligo labeling with amino groups directly linked to numerous fluors
BioLoop and AminoLoop can be used in FISH or other applications requiring labeled oligos
 
 

 

References

O'Keefe et al. Hum Mol Genet. 1996 Nov; 5(11):1793-1799

Pinkel. ISAC meeting Ashville, NC, March 1990

Tkachuk et al. ASHG meeting Cincinnati, OH, September 1990