This schematic diagram illustrates the basis for the GMD secretion assay. Each cell is encapsulated using (left). Using streptavidin as a bridge, biotinylated antibody specific for the secreted protein of interest is distributed throughout the GMD (magnified view). After a secretion incubation, frequently as short as 15 minutes, the secreted protein bound in the GMD is quantified by the addition of a second antibody which is fluorescently labeled, similar to sandwich assay format. Each GMD can be quantified and cells of interest can be isolated and recovered using FACS. The florescence intensity of GMD-associated fluorescence.  After sorting, cells typically outgrow CelBioGel GMDs or agarase can be used to digest the the matrix.

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